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| Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J.. |
| The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035 |
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| Marin,J.M.; Maluta,R.P.; Borges,C.A.; Beraldo,L.G.; Maesta,S.A.; Lemos,M.V.F.; Ruiz,U.S.; Ávila,F.A.; Rigobelo,E.C.. |
| Livestock manure may contain pathogenic microorganisms which pose a risk to the health of animal or humans if the manure is not adequately treated or disposed of. To determine the fate of Shiga toxigenic Escherichia coli (STEC) non O157 in composted manure from naturally colonized sheep, fresh manure was obtained from animals carrying bacterial cells with stx1/ stx2 genes. Two composting systems were used, aerated and non-aerated, and the experiments were done in Dracena city, São Paulo State. Every week, for seven weeks, one manure sample from six different points in both systems was collected and cultured to determine the presence of E. coli, the presence of the virulence genes in the cells, and also the susceptibility to 10 antimicrobial drugs. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial elimination; VTEC; Environment; Compost; Virulence gene. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601771 |
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| Lima,A.S.G.; Guidelli,A.M.; Abreu,I.L.; Lemos,M.V.F.. |
| PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: REP; ERIC; Crystal protein; Parasporal bodies. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017 |
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| Paiva,J.B.; Penha Filho,R.A.C.; Pereira,E.A.; Lemos,M.V.F.; Barrow,P.A.; Lovell,M.A.; Berchieri Jr,A.. |
| Salmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHIJ) operons in Salmonella Typhimurium (STM) encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO, TMAO, and nitrate, respectively. They are regulated in response to nitrate and oxygen availability and changes in cell growth rate. In this study mortality rates of chickens challenged with mutants of Salmonella Gallinarum, which were defective in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Gallinarum; Anaerobic genes; Poultry; Mutations. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400035 |
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